Cation Exchange Chromatography for Monoclonal Antibody (mAb) Separations INTRODUCTION Recombinant monoclonal antibodies (mAbs) have become a very important segment of protein drug therapeutics. Post-translational modifications including glycosylation, oxidation, deamidation and incomplete C-terminal. Ion exchange chromatography is one of the most frequently used techniques for purification of biomolecules and separates the molecules according to differences in their net surface charge.
Reversed Stage, Hydrophilic Connections and Regular Phase Chromatography CoIumns
Artwork CONSERVATION: Make use of OF CHROMATOGRAPHY lN
S.L. Vallance, inEncyclopedia of Separation Research, 2000Ion Exchange Chrómatography
lon exchange chromatography had been first used for the analysis of samples from works of art in 1969 when the successful analysis of antique and contemporary art individuals was reported. The eluted amino acids were discovered by optical denseness with ninhydrin, then the percentage amino acid structure was calculated for each test. Examples of gelatine, caséin, glair, tempera ánd even pet horn were characterized making use of this technique.
The make use of of ion éxchange chrómatography in this specific area is certainly difficult: the method of test preparation is certainly both extended and complex, pH gradients are hard to control exactly and the required sample size of color is fairly large when place into the context of a specimen to end up being removed from a useful work of art. Additionally, museums and galleries are notoriously brief of both money and area, thus specific single-purpose products is deemed unaffordable by many institutions.